ABSTRACT Introduction: According to the background of the healthy aging population and national fitness care, rapid development has begun in national sports in China. This development is the result of current research on the current scenario. Objective: Study the effects of exercise on the rehabilitation of middle-aged and elderly people with sports injuries. Methods: Twenty individuals were recruited for the experiment; the experimental group received the rehabilitation exercise protocol three times a week for four weeks, while the control group maintained the original lifestyle with no exercise or vigorous exercise. Results: It was found that there was no significant difference in height, weight, and body fat percentage after the aerobic exercise intervention training protocol (P>0.05), but there was a significant difference in BMI between the experimental group and the control group after the intervention training period (P<0.05). The VAS score of the experimental group before and after training decreased from 5.49 to 0.90, showing a significant effect, indicating a significant difference between the experimental group before and after training (P<0.05). Conclusion: The research shows that exercise positively affects the prevention and rehabilitation of sports injuries. The results obtained can help groups in national fitness. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMEM Introducción: De acuerdo con el trasfondo del envejecimiento saludable de la población y el cuidado de la forma física nacional, se ha iniciado un rápido desarrollo del deporte nacional chino. Este desarrollo es el resultado de la investigación en curso sobre el escenario actual. Objetivo: Estudiar los efectos del ejercicio en la rehabilitación de personas de mediana y avanzada edad con lesiones deportivas. Métodos: Veinte individuos fueron reclutados para el experimento, el grupo experimental recibió el protocolo con ejercicio de rehabilitación 3 veces por semana durante 4 semanas, mientras que el grupo de control mantuvo el estilo de vida original sin ejercicio o con ejercicio vigoroso. Resultados: Se observó que no había diferencias significativas en la estatura, el peso y el porcentaje de grasa corporal tras el protocolo de entrenamiento de intervención con ejercicio aeróbico (P>0,05); sin embargo, sí había diferencias significativas en el IMC entre el grupo experimental y el grupo de control tras el periodo de entrenamiento de intervención (P<0,05). La puntuación VAS del grupo experimental antes y después del entrenamiento disminuyó de 5,49 a 0,90, mostrando un efecto significativo, lo que indica que hubo una diferencia significativa entre el grupo experimental antes y después del entrenamiento (P<0,05). Conclusión: La investigación demuestra que el ejercicio tiene un efecto positivo en la prevención y rehabilitación de las lesiones deportivas. Los resultados obtenidos pueden ayudar a los grupos en la preparación física nacional. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

RESUMO Introdução: Segundo o contexto do envelhecimento saudável da população e dos cuidados da condição física nacional, iniciou-se um rápido desenvolvimento nos esportes nacionais chineses. Esse desenvolvimento é fruto de pesquisas atuais sobre o cenário atual. Objetivo: Estudar os efeitos do exercício físico na reabilitação de pessoas de meia-idade e idosas com lesões ocasionadas pelo esporte. Métodos: Foram recrutados 20 indivíduos para o experimento, o grupo experimental recebeu o protocolo com exercício de reabilitação 3 vezes por semana durante 4 semanas, enquanto o grupo de controle manteve o estilo de vida original sem exercícios ou com exercícios vigorosos. Resultados: Verificou-se que não houve diferença significativa em altura, peso e percentual de gordura corporal após o protocolo de treinamento de intervenção aeróbica de exercício (P>0,05); porém houve diferença significativa no IMC entre o grupo experimental e o grupo controle após o período de treinamento de intervenção (P<0,05). O escore VAS do grupo experimental antes e depois do treinamento diminuiu de 5,49 para 0,90, apresentando um efeito significativo, indicando que houve uma diferença significativa entre o grupo experimental antes e depois do treinamento (P<0,05). Conclusão: A pesquisa mostra que o exercício tem um efeito positivo na prevenção e na reabilitação de lesões esportivas. Os resultados obtidos podem ajudar grupos em aptidão física nacional. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

Abstract Astragaloside IV (AS-IV) has been shown to possess cardioprotective effect. However, the specific mechanism of AS-IV in myocardial infarction (MI) remains unclear. Our results showed that AS-IV intervention observably enhanced cell viability and reduced cell apoptosis, oxidative stress levels and inflammatory factor secretion. Expression of MIRT1 was significantly up-regulated in hypoxia-treated cells. In addition, ALCAM overexpression reversed the effects of AS-IV intervention on hypoxia-treated cell functions. MIRT1 facilitated Runx3 promoter methylation and its downregulated expression by recruiting DNA methyltransferase 3B (DNMT3B) to the Runx3 promoter region through binding with suppressor of Zeste 12 protein homolog (Suz12). Runx3 silencing reversed the effects of MIRT1 inhibition on hypoxia-treated cell functions. Betulinic acid suppressed the effects of AS-IV intervention on the behaviors of hypoxia-treated cells. AS-IV treatment improved the cardiac functions of mice with MI. Taken together, these findings demonstrated that AS-IV treatment inhibits DNMT3B-mediated Runx3 promoter methylation by restraining Suz12 expression and further blocks the NF-κB signaling pathway, and in turn improves the cardiac functions of mice with MI.

Abstract Triptolide is a natural active compound that has significant neuroprotective properties and shows promising effects in the treatment of Alzheimer’s disease (AD). Recent studies have shown that autophagy occurs in AD. In this study, we determined whether autophagy regulated by triptolide ameliorates neuronal death caused by amyloid-Beta1-42 (Aβ1-42). We examined the effects of triptolide on cell viability, autophagy, apoptosis, and the protein kinase B/mammalian target of the rapamysin/70 kDa ribosomal protein S6 kinase (Akt/mTOR/p70S6K) signaling pathway in PC12 cells. The results indicated that triptolide treatment exhibited a cytoprotective effect against cell injury induced by Aβ1-42. Triptolide also reduced apoptosis and enhanced cell survival by decreasing autophagosome accumulation and inducing autophagic degradation. Furthermore, our results also showed that activating the Akt/mTOR/p70S6K mechanism was one reason for the protection of triptolide. Triptolide treatment protected against Aβ1-42-induced cytotoxicity by decreasing autophagosome accumulation, and inducing autophagic degradation in PC12 cells. These findings also suggest that the reduction of autophagosome accumulation observed in triptolide-treated cells was Akt/mTOR/p70S6K pathway dependent. Overall, triptolide exhibits a neuron protective effect and this study provides new insight into AD prevention and treatment.

The outbreak of the 2019 novel coronavirus disease (COVID-19) has impacted the mental health of healthcare providers at the frontline. Therefore, we conducted this study to estimate the prevalence rate of anxiety and insomnia and identify associated risk factors among healthcare workers in Jilin, China, during the period from January 25 to February 25, 2020. Zung's Self-Reported Anxiety Scale (SAS) and the Insomnia Severity Index (ISI) scale were used to diagnose anxiety and insomnia, respectively. Associated risk factors were identified through a multivariate logistic regression model. A total of 300 healthcare workers were invited and 236 completed the study. Of them, 234 (99.15%) were medical workers, 197 (83.47%) were working at frontline departments, and 159 (67.37%) were fighting against COVID-19. Fifty-seven respondents (24.15%) had anxiety (SAS index score ≥45) and 94 (39.83%) had insomnia (ISI score ≥8). Based on the multivariate analysis, contact with people from Hubei province during work (no vs not clear) [OR=0.25, 95%CI: 0.10-0.61] and personal protective equipment (PPE) (not in place vs in place) [OR=6.22, 95%CI: 2.23-17.40] were significantly correlated with anxiety. PPE (not in place vs in place) was the only significant risk factor of insomnia [OR=10.56, 95%CI: 4.00-27.87]. The prevalence of anxiety and insomnia was high in our study, reflecting the psychological impact of COVID-19 on healthcare workers. The unavailability of PPE in place was a significant risk factor of both anxiety and insomnia.